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This reduction of glutathione in the mitochondria and cell cytosol results in decreased excretion of reactive oxygen species and peroxynitrite[30]

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The isolated fetal thymic lobes (E15.5) were cultured on Nucleopore filters (Whatman) in R10 medium, which consists of RPMI 1640 (Fujifilm Wako), 10% fetal bovine serum (Equitech-Bio), 2 mM L-glutamine (Fujifilm Wako), 100 U/mL penicillin (Banyu Pharmaceutical), 100 µg/mL streptomycin (Meiji Seika Pharma Co., Ltd.), 50 µM 2-mercaptoethanol (Fujifilm Wako) and 1.35 mM 2′-deoxyguanosine (Sigma-Aldrich) for 4 days